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(1) Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.[TOP]

Pubmed ID :30677020
Publication Date : //
Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening.

Authors : Sima Michal, Ferencova Blanka, Bhattacharyya Tapan, Miles Michael A, Litvinov Sergey V, Hailu Asrat, Baneth Gad, Volf Petr,

(2) Detection of Specific Antibody Reactivity to Larval Excretory-secretory Antigens in Asthmatic Patients (5-15 Years).[TOP]

Pubmed ID :27857820
Publication Date : //
Humans act as an intermediate host for and . Toxocara may be an important risk factor for asthma in humans. The aim of the present study was to evaluate immunoglobulin G (IgG) anti- canis antibody, using enzyme-linked immunosorbent assay () in asthmatic patients (aged 5-15 years), referring to a clinic of pulmonary diseases in Arak, Iran.

Authors : Mosayebi Mahdi, Moini Latif, Hajihossein Reza, Didehdar Mojtaba, Eslamirad Zahra,

(3) [TOP]

Pubmed ID :
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Authors :

(4) [TOP]

Pubmed ID :
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Authors :

(5) Serological evidence of hepatitis E virus infection in different animal species from the Southeast of Brazil.[TOP]

Pubmed ID :16021297
Publication Date : //
Serological evidence of hepatitis E virus infection (HEV) has been observed in both humans and different animal species living in non-endemic areas, suggesting that animals could be important reservoir for virus transmission to man. Antibodies to HEV have been detected in some Brazilian population groups. Nevertheless, sporadic cases of acute HEV infection have never been reported. We collected 271 serum samples from several domestic animals and also from pig handlers from Southeast of Brazil in order to investigate the seroprevalence of HEV infection. Anti-HEV IgG was detected in cows (1.42%), dogs (6.97%), chickens (20%), swines (24.3%), and rodents (50%), as well as in pig handlers (6.3%). The recognition of swine HEV infections in pigs in many countries of the world led us to investigate a larger sample of pigs (n = 357) from the same Brazilian region with ages ranging from 1 to > 25 weeks. IgG anti-HEV was detected in 100% of 7-day old pigs. Following a gradual decline between weeks 2 and 8 (probably due to loss of maternal IgG), the prevalence then steady increased until it reached 97.3% of animals older than 25 weeks. Besides the detection of anti-HEV antibodies in different animal species, the results showed that swine HEV infection seems to be almost universal within this Brazilian pig population. This is the first report that shows evidences of HEV circulation in Brazilian animal species and pig handlers.

Authors : Vitral Cláudia L, Pinto Marcelo A, Lewis-Ximenez Lia L, Khudyakov Yuri E, dos Santos Débora R, Gaspar Ana Maria C,

(6) Physico-chemical and antigenic characterization of unconventional heavy chain antibodies of Indian desert camel (Camelus dromedarius L.).[TOP]

Pubmed ID :22900358
Publication Date : //
Heavy chain antibodies (HCAbs) of IgG2 and IgG3 subtypes were purified from the sera of Indian desert camel (Camelus dromedarius L.) by ammonium sulphate precipitation, followed by ion-exchange chromatography on DEAE-cellulose and affinity chromatography on protein A-sepharose and protein G-sepharose, and characterized by SDS-polyacrylamide gel electrophoresis, agar gel immunodiffusion (AGID), counter-immunoelectrophoresis (CIEP), immunoelectrophoresis (IEP), ELISA and immunoblotting. IgG2 and IgG3 were found to have molecular mass 46.77 kDa and 43.65 kDa, respectively by SDS-PAGE under reducing conditions. They migrated in beta-region in IEP and could be detected in CIEP, because of being more negatively charged and smaller size. Anti-camel IgG3 cross-reacted in AGID, ELISA and immunoblotting with IgGs of pig and ruminants (cattle, buffalo, sheep and goat), but not with immunoglobulins from horse, dog, guinea pigs, mice, fish, poultry and human. The present findings suggest close antigenic relationship of camels with pigs and ruminants.

Authors : Sehrawat Sharvan, Singh Ajit,

(7) Echinococcus granulosus: a seroepidemiological survey in northern Israel using an enzyme-linked immunosorbent assay.[TOP]

Pubmed ID :9463658
Publication Date : //
Following an intensive health education programme, 8651 finger-prick blood samples, 4122 from a predominantly adult group attending a primary care clinic and 4529 from schoolchildren, were collected in Tamra, northern Israel. An enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) was used to detect anti-Echinococcus granulosus antibodies, using both crude and purified antigens. The seroprevalence in the adult group was 0.48% (20/4122); optical density values were 0.1-0.14 in 10 subjects, 0.15-0.19 in 9, and > or = 0.2 in one; prevalences did not differ significantly between males and females or among age groups. Twenty-six of the schoolchildren (0.57%) were seropositive, 23 with optical densities of 0.1-0.14, one of 0.15-0.19, and 2 > or = 0.2. A high correlation was observed between ELISA positivity and both positivity in the arc 5 immunoelectrophoresis test and the presence of a high titre in the indirect immunofluorescence assay. Cross reactivity was observed with sera from schistosomiasis and ancylostomiasis patients, using both crude and purified echinococcal antigens. The results indicated that the IgG ELISA, using both crude and purified antigens, was very useful for seroepidemiological screening for echinococcosis, and that this condition is an emerging disease in northern Israel.

Authors : el-On J, Khaleel E, Malsha Y, Nahmias J, Schantz P, Sneir R, Ben-Ismail R, Furth M, Hoida G,

(8) Comparative studies on streptococci of serological group G isolated from various origins.[TOP]

Pubmed ID :8976617
Publication Date : //
The streptococcal cultures used in the present study were isolated from dogs, bovines and humans and could be classified into Lancefield's serological group G. Most of the group G streptococci grew in fluid media as granular sediment with clear supernatant and formed compact colonies in soft agar. The majority of the group G streptococci from dogs and bovines displayed CAMP-like synergistic haemolytic activities on sheep blood agar, fermented lactose and salicin and produced the enzyme alpha-D-galactosidase. The group G streptococci from humans mainly fermented trehalose and produced the enzyme beta-D-glucuronidase. In addition, some of the group G streptococci reacted with type antigen X and R and two cultures with M6 specific antiserum. A positive opacity factor reaction could be observed with few group G streptococci isolated from dogs and bovines, but not with those from humans. In binding studies with 125I-labelled plasma proteins most of the cultures interacted with 125I-immunoglobulin G and 125I-albumin. Binding of 125I-IgG was more pronounced among group G streptococci isolated from humans. The determination of antibiotic susceptibility revealed that most of the group G streptococci were susceptible to bacitracin, cefoxitin, clindamycin, erythromycin, gentamicin, penicillin and sulfamethoxazole-trimethoprim. Some of the cultures were resistant to minocycline, neomycin and tetracycline. All this data clearly distinguished group G streptococci isolated from animals and humans and could additionally be used for individual characterization of this microorganism. This might be useful in epidemiological aspects and contribute to understanding infections caused by these bacteria.

Authors : Soedarmanto I, Lämmler C,

(9) Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are effected by the alternative complement pathway.[TOP]

Pubmed ID :7676614
Publication Date : //
Sea bream serum displayed bactericidal and hemolytic activities. These activities were depleted when serum was incubated with different activators of the alternative complement pathway (ACP). Ethylenediaminetetraacetic acid (EDTA) inhibited both the hemolytic and bactericidal activities, while ethyleneglycol-bis (B-aminoethyl ether)-N, N, N'-tetraacetic acid (EGTA) was not inhibitory. An antibody against the putative third component of sea bream component (C3) was produced. It was observed by immunoelectrophoresis that the sea bream C3 and human C3 migrated in the same position. Crossed immunoelectrophoresis showed that sea bream C3 exhibited a similar pattern of activation when compared with its human counterpart. The anti-sea bream C3 antibody inhibited both bactericidal and hemolytic activities. It was concluded that both serum actions were displayed by the ACP. The best conditions for the sea bream ACP titration were investigated. Of all mammal erythrocytes tested, rabbit erythrocytes (RaRBC) were found to be the best ACP activators and thus were used for the titration. Sea bream showed very high ACP titers when compared with those of mammals. Absorption of naturally occurring antibodies against rabbit RaRBC did not influence the ACP titers. Enzymatic removal of sialic acid from different mammalian erythrocytes increased the sensitivity of these cells to hemolysis mediated by the sea bream ACP.

Authors : Sunyer J O, Tort L,

(10) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.[TOP]

Pubmed ID :8116221
Publication Date : //
Twenty commercially available monoclonal antibodies (mAbs) raised against various human leucocyte surface antigens were tested on lymphocytes, monocytes and granulocytes from horse, pig, cow, sheep, goat, dog, mink, rabbit, rhesus monkey as well as man. Eight antibodies reacted with leucocytes from some of the animals. These were the antibodies against CD2, CD4, CD5, CD8, CD14, CD18, HLA-DR, and the HLA-ABC antigen. The CD18 antibody reacted with lymphocytes, monocytes and granulocytes from all animals tested except goat. The CD14 antibody reacted with monocytes from pig, cow, sheep, goat, dog, mink, rabbit and monkey and with granulocytes from pig and rabbit. The anti-HLA-ABC reacted with leucocytes from monkey and cow. Finally, the CD2, CD4, CD5 and CD8 antibodies reacted with leucocytes from monkey only.

Authors : Jacobsen C N, Aasted B, Broe M K, Petersen J L,