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DNotion Transfection Reagent _ 4 x 1.2ml

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[#2201410] DNotion Transfection Reagent _ 4 x 1.2ml

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(1) Antisense oligonucleotide targeting CD39 improves anti-tumor T cell immunity.[TOP]

Pubmed ID :30871609
Publication Date : //
Cancer cells are known to develop mechanisms to circumvent effective anti-tumor immunity. The two ectonucleotidases CD39 and CD73 are promising drug targets, as they act in concert to convert extracellular immune-stimulating ATP to adenosine. CD39 is expressed by different immune cell populations as well as cancer cells of different tumor types and supports the tumor in escaping immune recognition and destruction. Thus, increasing extracellular ATP and simultaneously reducing adenosine concentrations in the tumor can lead to effective anti-tumor immunity.

Authors : Kashyap Abhishek S, Thelemann Tamara, Klar Richard, Kallert Sandra M, Festag Julia, Buchi Melanie, Hinterwimmer Lisa, Schell Monika, Michel Sven, Jaschinski Frank, Zippelius Alfred,



(2) Selected lipid-based transfection reagents activate NF-κB and MAP kinases signaling pathways, induced cytokines mRNA expression in human THP-1 macrophage.[TOP]

Pubmed ID :30853376
Publication Date : //
In the present study, we evaluated, under transient transfection conditions, five different cationic lipid-based transfection reagents on the activation of NF-κB, MAP kinases signaling pathways and induction of cytokines expression. We found that the reagents studied differentially regulated the NF-κB and the MAP kinases signaling pathways in the human THP-1 macrophage. Additionally, mRNA expression levels of the cytokines, IL-1β and TNF-α in THP-1 macrophage were also induced by selected test reagents. Hence, careful selection of cationic lipid-based transfection reagent for transient transfection is warranted, especially in studies of gene expression and function mediated through NF-kB- and MAP kinases-mediated pathways.

Authors : Choe Uyory, Yu Liangli, Wang Thomas T Y,



(3) The anti-tumor growth effect of a novel agent DMAMCL in rhabdomyosarcoma in vitro and in vivo.[TOP]

Pubmed ID :30850026
Publication Date : //
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children with poor survival. New treatment approaches are urgently needed to improve treatment efficacy in RMS patients. DMAMCL is a novel agent from Asteraceae family that has been tested in phase I clinical trials in adult glioma in Australia.

Authors : Xu Ning, Hua Zhongyan, Ba Gen, Zhang Simeng, Liu Zhihui, Thiele Carol J, Li Zhijie,



(4) Generation of CD19-Targeted Chimeric Antigen Receptor T Cells.[TOP]

Pubmed ID :30821155
Publication Date : //
Current advancements in the field of chimeric antigen receptor (CAR) therapy, particularly U.S. FDA approval of Kymriah and Yescarta, heralds a new era of cancer treatment. This rapid progress in technology has urged more countries and institutions to keep pace with the fast-growing and developing technology of producing CAR T cell-based therapies in the race to develop new cancer-targeting drugs. Hence, for stepping in line with global advances and to pave the way for subsequent preclinical and clinical studies, we have established a development protocol for a cancer-targeting CAR T cell; we have chosen CD19 CAR T cell as a well-defined model to set-up T cell expansion, activation, and viral transduction as the prerequisites for diverse CAR T cell therapies.

Authors : Kiani Jafar, Naderi Mahmood, Torabi-Rahvar Monireh, Ranjbar Azam, Aghayan Hamid-Reza, Janzamin Ehsan, Ahmadbeigi Naser,



(5) FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.[TOP]

Pubmed ID :30736838
Publication Date : //
Angiogenesis plays a critical role in the progression of glioma. Previous studies have indicated that RNA-binding proteins (RBPs) interact with RNAs and participate in the regulation of the malignant behaviors of tumors. As a type of endogenous non-coding RNAs, circular RNAs (circRNAs) are abnormally expressed in various cancers and are involved in diverse tumorigeneses including angiogenesis.

Authors : He Zhenwei, Ruan Xuelei, Liu Xiaobai, Zheng Jian, Liu Yunhui, Liu Libo, Ma Jun, Shao Lianqi, Wang Di, Shen Shuyuan, Yang Chunqing, Xue Yixue,



(6) Autophagy inhibitor Vacuolin-1 interferes with lipid-based small interference RNA delivery.[TOP]

Pubmed ID :30732855
Publication Date : //
Autophagy and endocytosis are important pathways regulating macromolecule recycling and regeneration. Small molecule inhibitors are utilized to modulate these pathways and to treat autophagy-related diseases. Vacuolin-1 is a small molecule that can potently and reversibly inhibit autophagy by activating Rab5. In addition, Vacuolin-1 can be applied to inhibit exocytosis in a variety of cell types. Here we report that Vacuolin-1 significantly reduces small interference RNA (siRNA)-mediated gene silencing delivered by liposome transfection reagent or lipid nanoparticles in Hela cells. Vacuolin-1 exhibits the strongest inhibition effect among a few autophagy inhibitors including Chloroquine, Wortmannin, and Bafilomycin A1. We found that siRNAs are over-accumulated intracellularly and colocalized with a late endosome marker Rab7 in Vacuolin-1 treated cells, suggesting Vacuolin-1 inhibits the cytoplasmic release of lipid siRNA complexes from late endosomes. We propose that Vacuolin-1 could potentially be used to control the effects of lipid nanoparticle-based RNAi and gene therapy drugs.

Authors : Li Tianzhong, Yue Jianbo, Huang Linfeng, Yang Mengsu,



(7) [Inhibition of autophagy suppresses osteogenic differentiation of stem cells from apical papilla].[TOP]

Pubmed ID :30692075
Publication Date : //
To investigate the effects of autophagy on osteogenic differentiation of stem cells from the apical papilla (SCAPs) in the presence of tumor necrosis factor- (TNF-) stimulation .

Authors : Huang Ying, Xiong Huacui, Chen Ke, Zhu Xiaobin, Yin Xiaoping, Liang Yun, Luo Wei, Lei Qiyin,



(8) Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents.[TOP]

Pubmed ID :30686003
Publication Date : //
In this study, the optimal dose of Lipofectamine 3000 and Turbofect to transfect adherent cell lines such as CHO-K1 and HEK293 cells in comparison with non-adherent H9T-cells with pEGFP-N1 and pCDH was identified.

Authors : Rahimi P, Mobarakeh V Iranpur, Kamalzare S, SajadianFard F, Vahabpour R, Zabihollahi R,



(9) Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway.[TOP]

Pubmed ID :30670041
Publication Date : //
We developed a non-viral vector, a combination of HIV-1 Tat peptide modified with histidine and cysteine (mTat) and polyethylenimine, jetPEI (PEI), displaying the high efficiency of plasmid DNA transfection with little toxicity. Since the highest efficiency of INTERFERin (INT), a cationic amphiphilic lipid-based reagent, for small interfering RNA (siRNA) transfection among six commercial reagents was shown, we hypothesized that combining mTat/PEI with INT would improve transfection efficiency of siRNA delivery. To elucidate the efficacy of the hybrid vector for siRNA silencing, β-actin expression was measured after siRNA β-actin was transfected with mTat/PEI/INT or other vectors in HSC-3 human oral squamous carcinoma cells.

Authors : Kasai Hironori, Inoue Kenji, Imamura Kentaro, Yuvienco Carlo, Montclare Jin K, Yamano Seiichi,



(10) Engineering of a bioluminescent probe for imaging nitroxyl in live cells and mice.[TOP]

Pubmed ID :30664144
Publication Date : //
A bioluminescent probe, BP-HNO, which exhibits a turn-on response to nitroxyl with high sensitivity and selectivity, is reported for the first time in this work. BP-HNO is free from the interference of biological autofluorescence to afford a high signal-to-noise ratio for bioimaging, and was successfully applied to imaging nitroxyl in live cells and mice.

Authors : Li Jun-Bin, Wang Qianqian, Liu Hong-Wen, Yin Xia, Hu Xiao-Xiao, Yuan Lin, Zhang Xiao-Bing,