pET28a Vectors
The pET-28a-c(+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered… Read more »
Author Archives: Hunter
Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome
The fish insulin/insulin-like progress issue (IGF) pathway has weak management over carbohydrate metabolism. To know the molecular foundation for the metabolic variety, we characterised the forkhead field transcription issue O1A… Read more »
Cloning short DNA into plasmids by one-step PCR
Background: Plasmid building of small fragments of curiosity (similar to insertion of small fragment marker genes, expression of shRNA, siRNA, and many others) is the idea of many biomolecular experiments. Right… Read more »
In vivo DNA assembly using common laboratory bacteria: a re-emerging tool to simplify molecular cloning.
Molecular cloning is a cornerstone of biomedical, biotechnological and artificial biology analysis. As such, improved cloning methodologies can considerably advance the velocity and price of analysis tasks. Whereas present standard… Read more »
Exonuclease III (XthA) Enforces In Vivo DNA Cloning of Escherichia coli To Create Cohesive Ends.
Escherichia coli has a capability to assemble DNA fragments with homologous overlapping sequences of 15 to 40 bp at every finish. A number of modified protocols have already been reported to… Read more »
ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes.
The exponentially rising volumes of DNA sequence knowledge spotlight the necessity for brand new DNA cloning strategies to discover the brand new data. Right here, we describe ‘ExoCET’ (Exonuclease Mixed… Read more »
FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology.
Present challenges within the research and biotechnological exploitation of filamentous fungi are the optimization of DNA cloning and fungal genetic transformation past mannequin fungi, the open alternate of ready-to-use and… Read more »
Transient binding and jumping dynamics of p53 along DNA revealed by sub-millisecond
DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975)… Read more »
Changes of Circulating Tumor DNA in the Plasma of Rectal
Sizing Up Extracellular DNA: Immediate Chromatin Discharge From Cells When Positioned in Serum-Free Circumstances How do you wash cells? Three out of 4 of our colleagues use experimental procedures throughout… Read more »
DNA injury and peripheral blood lymphocytes in combating the oxidative stress
Protecting impact of leaf extract of Abutilon indicum on DNA injury and peripheral blood lymphocytes in combating the oxidative stress The present analysis explores in vitro antioxidant traits, radiation-induced DNA injury safety and quenching results… Read more »