Cloning short DNA into plasmids by one-step PCR

Cloning short DNA into plasmids by one-step PCR
Background: Plasmid building of small fragments of curiosity (similar to insertion of small fragment marker genes, expression of shRNA, siRNA, and many others) is the idea of many biomolecular experiments. Right here, we describe a technique to clone quick DNA into vectors by polymerase chain response (PCR), named one-step PCR cloning. Our methodology makes use of PCR to amplify your complete round plasmid. The PCR was carried out by the primers containing the gene of quick DNA with overlapping sequences between 10-15 bp. The PCR merchandise had been then remodeled into E. coli and cyclized by homologous recombination in vivo.
Strategies: The pEGFP-N1-HA plasmid was constructed by one-step PCR and transformation. Cells had been transfected with pEGFP-N1-HA and pEGFP-N1 plasmid utilizing TurboFect transfection reagent. Protein expression was detected by western blotting and the HA-GFP fusion protein was detected by confocal microscopy.
Outcomes: The pEGFP-N1-HA plasmid was efficiently constructed and HA expression in cells.
Conclusions: Free from the restrictions of restriction enzyme websites and omitting the ligation course of, our methodology provides a versatile and economical possibility of plasmid building.
Key factors: Vital findings of the research A technique to clone quick DNA into plasmids was discovered. What this research provides Our research supplies a versatile and economical choice to clone quick DNA into plasmids.

Selective isolation of huge segments from particular person microbial genomes and environmental DNA samples utilizing transformation-associated recombination cloning in yeast.

Right here, we describe an extension of our unique transformation-associated recombination (TAR) cloning protocol, enabling selective isolation of DNA segments from microbial genomes. The approach is predicated on the beforehand described TAR cloning process developed for isolation of a fascinating area from mammalian genomes which are enriched in autonomously replicating sequence (ARS)-like sequences, components that operate because the origin of replication in yeast. Such sequences aren’t widespread in microbial genomes. On this Protocol Extension, an ARS is inserted into the TAR vector together with a counter-selectable marker, permitting for choice of cloning occasions towards vector circularization.
Pre-treatment of microbial DNA with CRISPR-Cas9 to generate double-stranded breaks close to the focused sequences tremendously will increase the yield of region-positive colonies. Compared to different accessible strategies, this Protocol Extension permits selective isolation of any area from microbial genomes in addition to from environmental DNA samples. Your complete process could be accomplished in 10 d. Generally used and nicely accepted approaches are missing for site-directed modification of adenoviral vectors. Right here, we try to introduce an easy-to-implement technique for such objective with an instance of creating a replication competent adenoviral vector system from pKAd5 plasmid, an infectious clone of human adenovirus 5 (HAdV-5). PCR merchandise of GFP expression cassette and plasmid spine had been fused with the EcoRI/NdeI-digested fragment of pKAd5 to generate a modified intermediate plasmid pMDXE3GA by DNA meeting.
NdeI-digested fragment of pMDXE3GA was introduced again to pKAd5 to kind the adenoviral plasmid pKAd5XE3GA by restriction-ligation cloning. Recombinant adenovirus HAdV5-XE3GA was rescued, amplified and purified. The expression of GFP and the propagation of virus in adherent HEp-2 and suspension Ok562 cells had been investigated. Expression of goal gene was considerably enhanced in each cell traces contaminated with HAdV5-XE3GA as a result of virus replication.
 Cloning short DNA into plasmids by one-step PCR

Cloning short DNA into plasmids by one-step PCR

Common Template-Assisted, Cloning-free Methodology for the Technology of Small RNA-Expressing Dumbbell-Formed DNA Vectors.

Dumbbell-shaped DNA minimal vectors symbolize genetic vectors solely composed of the gene expression cassette of curiosity and terminal closing loop buildings. Dumbbell vectors for small hairpin RNA or microRNA expression are extraordinarily small-sized, which is advantageous with regard to mobile supply and nuclear diffusion. Standard methods for the era of small RNA-expressing dumbbell vectors require cloning of a respective plasmid vector, which is subsequently used for dumbbell manufacturing. Right here, we current a novel cloning-free methodology for the era of small RNA-expressing dumbbell vectors that additionally doesn’t require any restriction endonucleases.
This new PCR-based methodology makes use of a common DNA template comprising an inverted repeat of the minimal H1 promoter and the miR-30 stem. The sequences coding for small RNA expression are launched by the PCR primers. Dumbbells are shaped by denaturing and reannealing of the PCR product and are covalently closed utilizing ssDNA ligase. The brand new protocol generates plus- and/or minus-strand dumbbells, each of which had been proven to set off environment friendly goal gene knockdown.

Cytokeratin, Pan; Clones AE1 & AE3 (Concentrate)

A00152-C.1 0.1 ml
EUR 138

Chromogranin A; Clones LK2H10 & PHE5 (Concentrate)

A00160-C 1 ml
EUR 488

Chromogranin A; Clones LK2H10 & PHE5 (Concentrate)

A00160-C.1 0.1 ml
EUR 136

Multiple Endocrine Neoplasia I Antibody

20-abx113892
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Multiple Line Reader

LFRB-001 Each
EUR 3916.66
  • Product Category: Lateral-Flow Readers and Equipment
Description: Multiple Line Reader is a portable universal lateral-flow reader that is based on colour change method.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

20-abx002804
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

20-abx212396
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

20-abx212881
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 2 (EXTL2) Antibody

20-abx112417
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

20-abx112418
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

abx036241-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 2 (EXTL2) Antibody

abx036893-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Multiple Endocrine Neoplasia I (MEN1) Antibody

20-abx128411
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

20-abx109769
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 1 (EXTL1) Antibody

abx030322-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 1 (EXTL1) Antibody

abx030322-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

abx034206-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

abx034206-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 2 (EXTL2) Antibody

abx034288-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 2 (EXTL2) Antibody

abx034288-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 2 (EXTL2) Antibody

abx232911-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody

abx232912-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Leukemia - Multiple Myeloma Exosome

P141-MM NULL
EUR 0

Recombinant HCV Multiple Antigen

VAng-Lsx0198-1mg 1 mg
EUR 2800
Description: HCV Multiple Antigen, recombinant protein from E. coli.

Anti-HPV 6 L1 monoclonal antibody (6 clones per set)

CABT-B8803 20 ug× 6 clones
EUR 1677

Anti-HPV 11 L1 monoclonal antibody (6 clones per set)

CABT-B8804 20 ug× 6 clones
EUR 1677

Anti-HPV 16 L1 monoclonal antibody (6 clones per set)

CABT-B8805 20 ug× 6 clones
EUR 1677

Anti-HPV 18 L1 monoclonal antibody (6 clones per set)

CABT-B8806 20 ug× 6 clones
EUR 1677

Anti-HPV 31 L1 monoclonal antibody (6 clones per set)

CABT-B8807 20 ug× 6 clones
EUR 1677

Anti-HPV 33 L1 monoclonal antibody (6 clones per set)

CABT-B8808 20 ug× 6 clones
EUR 1677

Anti-HPV 45 L1 monoclonal antibody (6 clones per set)

CABT-B8809 20 ug× 6 clones
EUR 1677

Anti-HPV 52 L1 monoclonal antibody (6 clones per set)

CABT-B8810 20 ug× 6 clones
EUR 1677

Anti-HPV 58 L1 monoclonal antibody (6 clones per set)

CABT-B8811 20 ug× 6 clones
EUR 1677

p53 Cocktail; Clones Pab 1801 plus DO-7

A00093 6 ml
EUR 282

p53 Cocktail; Clones Pab 1801 plus DO-7

A00093.0025 25 ml
EUR 725

Napsin A; Clones NAPSA/1238 & NAPSA/1239 (Concentrate)

A00131-C 1 ml
EUR 488

Napsin A; Clones NAPSA/1238 & NAPSA/1239 (Concentrate)

A00131-C.1 0.1 ml
EUR 136

MART-1; Clones M2-7C10 & M2-9E3 (Concentrate)

A00133-C 1 ml
EUR 484

Cytokeratin, Pan; Clones AE1 & AE3 (Ready-To-Use)

A00152-0002 2 ml
EUR 104

Cytokeratin, Pan; Clones AE1 & AE3 (Ready-To-Use)

A00152-0007 7 ml
EUR 183

Cytokeratin, Pan; Clones AE1 & AE3 (Ready-To-Use)

A00152-0025 25 ml
EUR 449

Chromogranin A; Clones LK2H10 & PHE5 (Ready-To-Use)

A00160-0002 2 ml
EUR 101

Chromogranin A; Clones LK2H10 & PHE5 (Ready-To-Use)

A00160-0007 7 ml
EUR 178

Chromogranin A; Clones LK2H10 & PHE5 (Ready-To-Use)

A00160-0025 25 ml
EUR 435

p53 Cocktail; Clones Pab 1801 plus DO-7

A20093 2 ml
EUR 165

Multiple EGF Like Domains 9 (MEGF9) Antibody

20-abx007525
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

abx027366-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

abx027366-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

abx028250-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

abx028250-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 8 (EGFL8) Antibody

20-abx213757
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 8 (MEGF8) Antibody

abx215087-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody (Biotin)

20-abx105397
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody (FITC)

20-abx106816
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody (HRP)

20-abx108235
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Rna Binding Protein With Multiple Splicing Antibody

20-abx115330
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exostoses (Multiple)-Like 3 (EXTL3) Antibody Pair

abx117473-1pair5x96wellplates 1 pair (5x96 well plates)
EUR 1010
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

20-abx126159
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 6 (EGFL6) Antibody

abx036838-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

abx038060-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

20-abx133721
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

20-abx109332
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 8 (EGFL8) Antibody

abx122807-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 7 (EGFL7) Antibody

abx145646-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

20-abx014606
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody

20-abx014607
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 8 (EGFL8) Antibody

20-abx241508
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (Minpp1) Antibody

20-abx319662
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (Minpp1) Antibody

20-abx319666
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple inositol polyphosphate phosphatase 1 (MINPP1) Antibody

abx331046-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

abx331804-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

20-abx327823
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple inositol polyphosphate phosphatase 1 (MINPP1) Antibody

20-abx328388
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 8 (MEGF8) Antibody

20-abx324529
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple EGF Like Domains 9 (MEGF9) Antibody

20-abx338519
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 7 (EGFL7) Antibody

abx432640-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

EGF Like Domain Multiple 6 (EGFL6) Antibody

20-abx302793
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

EGF Like Domain Multiple 7 (EGFL7) Antibody

abx232666-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Multiple endocrine neoplasia 1 (Men1) polyclonal antibody

ABP-PAB-10831 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Multiple endocrine neoplasia 1 (MEN1) polyclonal antibody

ABP-PAB-10832 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

MAP kinase fragment [Multiple species]

A1079-1 1 mg
EUR 108
Description: This Mitogen-activated protein kinase (MAP kinase) fragment has a peptide sequence of Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu.The MAP kinases are serine/threonine-specific protein kinasesbelonging to the CMGC (CDK/MAPK/GSK3/CLK) kinase group.

MAP kinase fragment [Multiple species]

A1079-10 10 mg
EUR 340
Description: This Mitogen-activated protein kinase (MAP kinase) fragment has a peptide sequence of Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu.The MAP kinases are serine/threonine-specific protein kinasesbelonging to the CMGC (CDK/MAPK/GSK3/CLK) kinase group.

MAP kinase fragment [Multiple species]

A1079-25 25 mg
EUR 456
Description: This Mitogen-activated protein kinase (MAP kinase) fragment has a peptide sequence of Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu.The MAP kinases are serine/threonine-specific protein kinasesbelonging to the CMGC (CDK/MAPK/GSK3/CLK) kinase group.

MAP kinase fragment [Multiple species]

A1079-5 5 mg
EUR 224
Description: This Mitogen-activated protein kinase (MAP kinase) fragment has a peptide sequence of Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu.The MAP kinases are serine/threonine-specific protein kinasesbelonging to the CMGC (CDK/MAPK/GSK3/CLK) kinase group.

Leukemia - Multiple Myeloma Exosome RNA

P241-MM NULL
EUR 0

Cytokeratin 5,6 Cocktail; Clones EP42 & EP67 (Ready-To-Use)

A00141-0002 2 ml
EUR 268

Cytokeratin 5,6 Cocktail; Clones EP42 & EP67 (Ready-To-Use)

A00141-0007 7 ml
EUR 594

Cytokeratin 5,6 Cocktail; Clones EP42 & EP67 (Ready-To-Use)

A00141-0025 25 ml
EUR 1683

Multiple Coagulation Factor Deficiency Protein 2 (MCFD2) Antibody

abx025215-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Multiple Coagulation Factor Deficiency Protein 2 (MCFD2) Antibody

abx025215-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

RNA-Binding Protein With Multiple Splicing (RBPMS) Antibody

20-abx003350
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

EGF Like Domain Protein, Multiple 6 (EGFL6) Antibody

20-abx176223
  • EUR 1316.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

EGF Like Domain Protein, Multiple 7 (EGFL7) Antibody

20-abx176224
  • EUR 1344.00
  • EUR 634.00
  • 1 mg
  • 200 ug
  • Please enquire.

EGF Like Domain Protein, Multiple 7 (EGFL7) Antibody

20-abx212160
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

CUB And Sushi Multiple Domains 1 (CSMD1) Antibody

20-abx212966
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

CUB And Sushi Multiple Domains 1 (CSMD1) Antibody

20-abx212967
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody (Biotin)

20-abx105681
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1) Antibody (FITC)

20-abx107098
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Multiple inositol polyphosphate phosphatase 1 (MINPP1) Antibody (HRP)

20-abx108519
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
This methodology allows quick, low cost manufacturing of small RNA-expressing dumbbell vectors in a excessive throughput-compatible method for practical genomics screens or, as dumbbells aren’t liable to transgene silencing, for knockdown research in main cells.

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