DNA methylation standing of imprinted H19 and KvDMR1 genes in human placentas

cloning-expression

Cell Cycle Modifications, DNA Ploidy, and PTTG1 Gene Expression in HTLV-1 Sufferers

Human T-cell lymphotropic virus type-1 (HTLV-1) is a pathogenic retrovirus that’s related to grownup T-cell leukemia/lymphoma (ATL). Genetic instability is the hallmark of ATL. Cell cycle development is required for virus particle replica. HTLV-1 encoded Tax protein finally disrupts the mitotic spindle checkpoint, resulting in incorrect chromosome segregation, leading to aneuploidy.
Cell cycle abnormalities have been described in T cells transfected with HTLV-1 virus in vitro, however not in HTLV-1 asymptomatic carriers. PTTG1 and HTLV-1 viral protein Tax exhibit a cooperative remodeling exercise. Overexpressed PTTG1 ends in chromosome instability and aneuploidy, which has been recommended as a mechanism underlying PTTG1 remodeling exercise.
Right here we aimed to research cell cycle, DNA ploidy and PTTG1 mRNA expression in CD4+ and CD8+ T cells in wholesome topics (HS), HTLV-1 asymptomatic carriers and ATL sufferers. We now have recognized that HTLV-1 asymptomatic carriers have proven DNA aneuploidy and cell cycle arrest at cell cycle section G0/G1 in CD4+ T cells. CD8+ T cells of HTLV-1 asymptomatic carriers additionally demonstrated DNA aneuploidy however with out alteration in cell cycle.
In ATL, CD4+ and CD8+ T cells current a better variety of cells in cell cycle S-phase and PTTG1 overexpression. These research present perception into malignant transformation of HTLV-1 asymptomatic carriers to ATL sufferers.

DNA methylation standing of imprinted H19 and KvDMR1 genes in human placentas after conception utilizing assisted reproductive know-how

 

Background: Assisted reproductive applied sciences (ARTs), akin to in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), are thought to destabilize genomic imprints. Earlier research inspecting the affiliation between ART and aberrant DNA methylation have been inconclusive.
Technique: The DNA methylation standing of H19 and KvDMR1was in contrast between newborns conceived by means of ART and people conceived naturally to judge the protection of ART. Placental tissues from 6 full-term, naturally conceived pregnancies (no gestational comorbidities) and 6 full-term ART pregnancies (no gestational complication) have been collected. Genomic DNA (gDNA) and RNA have been extracted from each teams.
Actual-time PCR was used to research the mRNA expression ranges of H19 and KvDMR1 within the placenta for each teams. An entire-genome DNA methylation microarray was used to look at three placentas from full-term, naturally conceived pregnancies and three placentas from full-term IVF pregnancies.
Outcome: The expression degree of H19 within the IVF group was considerably larger than that within the pure being pregnant group, whereas the expression degree of KvDMR1 was considerably decrease within the ART group than within the pure being pregnant group. Additionally, human ART manipulation resulted in placental gDNA methylation modifications. Conclusion: Irregular methylation patterns have been detected in phenotypically regular phenotype conceived by ART, which can happen resulting from imprinting errors in sperm/oocyte cells or unintended effects of in vitro embryo tradition procedures. Additional investigation is important to find out whether or not imprinted gene expression and DNA methylation may be regulated by means of different mechanisms.
cloning-expression

cloning-expression

Proteus sp. RT PCR kit
RTq-H517-150D 150T
EUR 701
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Proteus sp. .
Proteus sp. RT PCR kit
RTq-H517-50D 50T
EUR 532.8
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Proteus sp. .
Proteus sp. One-Step PCR kit
Oneq-H517-100D 100T
EUR 747.4
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Proteus sp..
Proteus sp. One-Step PCR kit
Oneq-H517-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Proteus sp..
Proteus sp. One-Step PCR kit
Oneq-H517-50D 50T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Proteus sp..
Native Proteus sp. Glutamate Dehydrogenase (NADP-dependent)
DIA-196 5 KU
EUR 270

Retrospective overview of outcomes in sufferers with DNA-damage restore associated pancreatic most cancers

Background: Sufferers with DNA-damage response genes (DDR)-related pancreas most cancers (BRCA1/2 or different DNA-damage associated genes) might have improved outcomes secondary to elevated sensitivity to DNA-damaging medication (platinum chemotherapy/ poly ADP ribose polymerase (PARP)-inhibitors). Nonetheless, information is scarce pertaining to outcomes on this subset of sufferers. Our goal was to retrospectively determine DDR-related pancreas most cancers sufferers and report on scientific outcomes.
Strategies: Pancreas most cancers sufferers with a germline pathogenic variant in BRCA1/2 or different DDR gene have been recognized retrospectively by means of overview of medical information (medical genetics/oncology) and genetic testing outcomes at our establishment. Information concerning scientific outcomes, remedy acquired, and survival was subsequently extracted.
Outcomes: A complete of 11 sufferers with pancreas most cancershave beenrecognizedto hold a pathogenic DDR-variantBRCA1 (3), ATM (4), BRCA2 (2), PALB2 (1) and FANCC (1). 5 of those people had prior historical past of different cancers. Clinically these tumors have been localized (4), regionally superior (3), and metastatic (4) at analysis. 4 out of 11 sufferers have been nonetheless alive at time of information overview. Survival within the 7 sufferers who had died was 13.7, 140.0, 20.5, 22.3, 23.5, 25.8, and 111.5 months. All sufferers with superior illness had publicity to platinum chemotherapy.
Conclusions: Historic survival in sufferers with superior and metastatic pancreas most cancers is poor. Outcomes of this DDR-subset of sufferers do present considerably superior outcomes, seemingly secondary to publicity to platinum medication. This information, alongside different comparable cohorts, would favor the DDR-genes being a predictive marker with improved survival if uncoveredto thosemedication and the brand new class of medication, PARP-inhibitors.

Anti-Phospho-DNAM-1 (S329) antibody

STJ91074 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-DNAM-1 (S329).

CD226/DNAM-1 Antibody

AF4778 200ul
EUR 376
Description: CD226/DNAM-1 Antibody detects endogenous levels of CD226/DNAM-1.

CD226/DNAM-1 Antibody

AF0087 200ul
EUR 304
Description: CD226/DNAM-1 antibody detects endogenous levels of total CD226/DNAM-1.

DNAM-1 Polyclonal Antibody

ES4132-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4132-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

CD226 / DNAM-1 Antibody

20-abx119383
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNAM-1 Polyclonal Antibody

ABP53133-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human DNAM-1
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human DNAM-1
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human DNAM-1
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53533-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

CD226/DNAM- 1 Antibody

ABF0087 100 ug
EUR 438

DNAM-1 Polyclonal Antibody

41787-100ul 100ul
EUR 252

DNAM-1 Polyclonal Antibody

41787-50ul 50ul
EUR 187

DNAM-1 Polyclonal Antibody

40846-100ul 100ul
EUR 252

DNAM-1 Polyclonal Antibody

40846-50ul 50ul
EUR 187

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

CD226/DNAM-1 Blocking Peptide

AF4778-BP 1mg
EUR 195

DNAM-1 Polyclonal Conjugated Antibody

C40846 100ul
EUR 397

CD226/DNAM-1 Blocking Peptide

AF0087-BP 1mg
EUR 195

Phospho-CD226/DNAM-1 (Ser329) Antibody

AF4478 200ul
EUR 376
Description: CD226/DNAM-1 (Phospho-Ser329) Antibody detects endogenous levels of CD226/DNAM-1 only when phosphorylated at Ser329.

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329
  • Applications tips:
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-100ul 100ul
EUR 252

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-50ul 50ul
EUR 187

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Phospho-CD226/DNAM-1 (Ser329) Blocking Peptide

AF4478-BP 1mg
EUR 195

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-48T 48T
EUR 289

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-96T 96T
EUR 466

Human DNA methyltransferase,DNAM ELISA Kit

201-12-1975 96 tests
EUR 440
  • This DNA methyltransferase ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human DNA methyltransferase(DNAM)ELISA Kit

QY-E04972 96T
EUR 361

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-1mg 1mg
EUR 1318
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-500ug 500ug
EUR 963
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-50ug 50ug
EUR 278
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-1mg 1mg
EUR 1318
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-500ug 500ug
EUR 963
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-50ug 50ug
EUR 278
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

DNAM-1 (Phospho-Ser329) Polyclonal Polyclonal Conjugated Antibody

C12361 100ul
EUR 397

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-100g 100 µg
EUR 1013
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-1mg 1 mg
EUR 6402
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

Anti-Apaf-1 (human) Monoclonal Antibody (2E12)

M00889-1 100ug
EUR 432
Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human.

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-100g 100 µg
EUR 738
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-1mg 1 mg
EUR 4174
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-100g 100 µg
EUR 738
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-1mg 1 mg
EUR 4174
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-48T 48T
EUR 317

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-96T 96T
EUR 496

Rat DNA methyltransferase(DNAM)ELISA Kit

QY-E10847 96T
EUR 361

Mouse DNA methyltransferase(DNAM)ELISA Kit

QY-E20479 96T
EUR 361

Anti-PARP-1 Antibody

A00122-1 100ul
EUR 397
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse.

Anti-Presenilin 1 Antibody

A00138-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-PAI-1 Antibody

A00637-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat.

Anti-Flk-1 Antibody

A00901-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse.

Anti-EDG-1 Antibody

A01502-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat.

Anti-ROBO-1 Antibody

A01530-1 100ug
EUR 455
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Bag-1 Antibody

A02423-1 50 ul
EUR 397
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat.

Anti-TUB 1 Antibody

A02917-1 100ug/vial
EUR 294

Anti-Dok-1 Antibody

A03039-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat.

Anti-TFIIIB90-1 Antibody

A03761-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse.

Anti-Atrophin-1 Antibody

A03828-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-CNG-1 Antibody

A05494-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Periphilin 1 Antibody

A06996-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse.

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